Clipboard, Search History, and several other advanced features are temporarily unavailable. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. ; Fran, H.P. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Biomacromolecules 8, 2464-2475. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Skin barrier disruption by acetone: observations in a hairless mouse skin model. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Kasaai, M. R.; Arul, J.; Charlet, G. (2000). "Dextrans in Aqueous Solution. J. Braz. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. (2013). The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Table 2 compares the properties of nanoparticles coated with one or other polymer. Kasaai, Mohammad R. (2007). Int. Viscosity of Liquids. Appl Biochem Biotechnol. Arond L.H. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Keywords: FITC dextran; intestinal permeability; transit time. (2008). ; Weisz, J. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. 2013;43(4):287303. 2006;3(3):37385. Not for use in diagnostic procedures. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. European Polymer Journal, 37, 619-622. Masuelli, Martin Alberto (2013). Microporation was performed 2 times on each sample. Ahn, G.Y., Eo, HS., Kim, D. et al. Format), Citation-(EndNote Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Progress in Polymer Science, 30, 38-70. The above data are not useful because it can not be compared with those obtained in this work. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. Webof overall macromolecular geometry and hydrodynamic draining. Theory, Estimation, Experiment, and Data. H&E stained images were analyzed using Image J software. 2019;26(1):25261. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. The slope is a value and intercept is equal to the ln kMH value [47]. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Progr. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Banga AK. A solvent at this temperature is called a theta solvent [53]. ; De Jong, S.J. He, L.; Niemeyer, B. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. HSE and DK helped to porate the skin and to measure TEWL. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Exp Cell Res. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. California Privacy Statement, (1954). ; Rjumtsev, E.I. Pharmacol Pharm. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Epub 2022 Feb 2. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. 2004;25(9):155967. Nordmeier, Eckliard (1993). 20K groups (n=3, *: p<0.05, **: p<0.01). (1958). AATEX. Biomater Res 24, 22 (2020). ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. 2019;9(3-S):9726. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. These studies were carried out at the Hisko (Gunpo, Korea). Chemical penetration enhancers for transdermal drug delivery systems. ; Yevlampieva N.P. Journal of Chemical Education, 80(9), 1036-1038. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. The genus Weissella: taxonomy, ecology and biotechnological potential. Webdextran hydrodynamic radius. Part of These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. ; Hogan, M.P. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Biophysical Journal, 87, 4259-4270. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. Eremeeva, T. E.; Bykova, T. O. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. Amersham Biosciences, AA Edition, www.amershambiosciences.com. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Epub 2021 Feb 9. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Smith, David R.; Raymonda, John W. (1972). WebDextran 40 Pharmaceutical Quality. The authors declare that they have no competing interests. (2006). For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Intrinsic viscosity of bovine serum albumin conformers. The micropores of the human cadaver skin were regenerated according to the time. GYA primarily conducted research. ; Wood, P.J. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. All branched polysaccharide types could be described approximately by the hyperbranching theory. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Journal Argentine Chemical Society 100 (2013) 25-34. European. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich official website and that any information you provide is encrypted Advances in chemistry series Washington, DC: American Chemical Society., pp. American Journal of Food Science and Technology 2013, 1(3), 60-66. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. Accessibility Chen Rong Huei; Min Lang Tsai (1998). The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Environ Toxicol Pharmacol. Eng. 2003;89(2):31120. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Expert Rev Med Devices. Sung-Wook Choi. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Transdermal drug delivery: overcoming the skins barrier function. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Dextran Fractions. 2015;32(5):170413. Carbohydrate Polymers, 79, 380-390. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2).
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